Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
Identifieur interne : 000999 ( Main/Exploration ); précédent : 000998; suivant : 000A00Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
Auteurs : Mohamed S. Al-Ayed ; Ahmed M. Asaad ; Mohamed A. Qureshi ; Mohammed S. AmeenSource :
- Saudi Medical Journal [ 0379-5284 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN viral (analyse), ARN viral (analyse), Arabie saoudite (épidémiologie), Co-infection (virologie), Co-infection (épidémiologie), Enfant d'âge préscolaire, Femelle, Humains, Infections de l'appareil respiratoire (virologie), Infections de l'appareil respiratoire (épidémiologie), Maladies virales (virologie), Maladies virales (épidémiologie), Mâle, Nourrisson, Nouveau-né, RT-PCR, Réaction de polymérisation en chaine multiplex, Virus (génétique), Virus (isolement et purification), Épidémiologie moléculaire, Études transversales.
- MESH :
- analyse : ADN viral, ARN viral.
- génétique : Virus.
- isolement et purification : Virus.
- virologie : Co-infection, Infections de l'appareil respiratoire, Maladies virales.
- épidémiologie : Arabie saoudite, Co-infection, Infections de l'appareil respiratoire, Maladies virales.
- Enfant d'âge préscolaire, Femelle, Humains, Mâle, Nourrisson, Nouveau-né, RT-PCR, Réaction de polymérisation en chaine multiplex, Épidémiologie moléculaire, Études transversales.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Child, Preschool, Coinfection (epidemiology), Coinfection (virology), Cross-Sectional Studies, DNA, Viral (analysis), Female, Humans, Infant, Infant, Newborn, Male, Molecular Epidemiology, Multiplex Polymerase Chain Reaction, RNA, Viral (analysis), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction, Saudi Arabia (epidemiology), Virus Diseases (epidemiology), Virus Diseases (virology), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- chemical , analysis : DNA, Viral, RNA, Viral.
- geographic , epidemiology : Saudi Arabia.
- epidemiology : Coinfection, Respiratory Tract Infections, Virus Diseases.
- genetics : Viruses.
- isolation & purification : Viruses.
- virology : Coinfection, Respiratory Tract Infections, Virus Diseases.
- Child, Preschool, Cross-Sectional Studies, Female, Humans, Infant, Infant, Newborn, Male, Molecular Epidemiology, Multiplex Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.
In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.
A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.
Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.
Url:
PubMed: 25399211
PubMed Central: 4362149
Affiliations:
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Le document en format XML
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Al-Ayed</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M." last="Asaad">Ahmed M. Asaad</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A." last="Qureshi">Mohamed A. Qureshi</name></author><author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S." last="Ameen">Mohammed S. Ameen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Saudi Medical Journal</title><idno type="ISSN">0379-5284</idno><idno type="eISSN">1658-3175</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Child, Preschool</term><term>Coinfection (epidemiology)</term><term>Coinfection (virology)</term><term>Cross-Sectional Studies</term><term>DNA, Viral (analysis)</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Molecular Epidemiology</term><term>Multiplex Polymerase Chain Reaction</term><term>RNA, Viral (analysis)</term><term>Respiratory Tract Infections (epidemiology)</term><term>Respiratory Tract Infections (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Saudi Arabia (epidemiology)</term><term>Virus Diseases (epidemiology)</term><term>Virus Diseases (virology)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term><term>ARN viral (analyse)</term><term>Arabie saoudite (épidémiologie)</term><term>Co-infection (virologie)</term><term>Co-infection (épidémiologie)</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Infections de l'appareil respiratoire (épidémiologie)</term><term>Maladies virales (virologie)</term><term>Maladies virales (épidémiologie)</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine multiplex</term><term>Virus (génétique)</term><term>Virus (isolement et purification)</term><term>Épidémiologie moléculaire</term><term>Études transversales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coinfection</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Co-infection</term><term>Infections de l'appareil respiratoire</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coinfection</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Arabie saoudite</term><term>Co-infection</term><term>Infections de l'appareil respiratoire</term><term>Maladies virales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Child, Preschool</term><term>Cross-Sectional Studies</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Molecular Epidemiology</term><term>Multiplex Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine multiplex</term><term>Épidémiologie moléculaire</term><term>Études transversales</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Objectives:</title><p>To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</p></sec><sec id="st2"><title>Methods:</title><p>In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.</p></sec><sec id="st3"><title>Results:</title><p>A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.</p></sec><sec id="st4"><title>Conclusion</title><p>Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bellos, A" uniqKey="Bellos A">A Bellos</name></author><author><name sortKey="Mulholland, K" uniqKey="Mulholland K">K Mulholland</name></author><author><name sortKey="O Rien, Kl" uniqKey="O Rien K">KL O’Brien</name></author><author><name sortKey="Qazi, Sa" uniqKey="Qazi S">SA Qazi</name></author><author><name sortKey="Gayer, M" uniqKey="Gayer M">M Gayer</name></author><author><name sortKey="Checchi, F" uniqKey="Checchi F">F Checchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Der Zalm, Mm" uniqKey="Der Zalm M">MM der Zalm</name></author><author><name sortKey="Van Ewijk, Be" uniqKey="Van Ewijk B">BE van 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uniqKey="Koekkoek S">SM Koekkoek</name></author><author><name sortKey="Visser, Ce" uniqKey="Visser C">CE Visser</name></author><author><name sortKey="Pajkrt, D" uniqKey="Pajkrt D">D Pajkrt</name></author><author><name sortKey="Molenkamp, R" uniqKey="Molenkamp R">R Molenkamp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Garcia, Ml" uniqKey="Garcia Garcia M">ML García-García</name></author><author><name sortKey="Calvo, C" uniqKey="Calvo C">C Calvo</name></author><author><name sortKey="Falc N, A" uniqKey="Falc N A">A Falcón</name></author><author><name sortKey="Pozo, F" uniqKey="Pozo F">F Pozo</name></author><author><name sortKey="Perez Bre A, P" uniqKey="Perez Bre A P">P Pérez-Breña</name></author><author><name sortKey="De Cea, Jm" uniqKey="De Cea J">JM De Cea</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tregoning, Js" uniqKey="Tregoning J">JS Tregoning</name></author><author><name sortKey="Schwarze, J" 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Fouchier</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S." last="Al-Ayed">Mohamed S. Al-Ayed</name><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S." last="Ameen">Mohammed S. Ameen</name><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M." last="Asaad">Ahmed M. Asaad</name><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A." last="Qureshi">Mohamed A. Qureshi</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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